Detailed view for Tb927.7.7040

Basic information

TDR Targets ID: 18127
Trypanosoma brucei, methylthioadenosine phosphorylase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2593 | Length (AA): 309 | MW (Da): 33443 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01048   Phosphorylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0017061   S-methyl-5-thioadenosine phosphorylase activity  
GO:0016763   transferase activity, transferring pentosyl groups  
GO:0003824   catalytic activity  
GO:0009116   nucleoside metabolic process  

Metabolic Pathways

Cysteine and methionine metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 283 1v4n (A) 7 271 43.00 0 1 1.43497 -0.86
12 302 4glj (A) 15 297 43.00 0 1 1.51875 -0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128424)

Species Accession Gene Product
Brugia malayi Bm1_42810   MTAP
Candida albicans CaO19.14200   ADH2 expression
Candida albicans CaO19.6938   ADH2 expression
Caenorhabditis elegans CELE_B0228.7   Protein B0228.7
Drosophila melanogaster Dmel_CG4802   Methylthioadenosine phosphorylase ortholog
Echinococcus granulosus EgrG_000622900   methylthioadenosine phosphorylase
Echinococcus multilocularis EmuJ_000622900   methylthioadenosine phosphorylase
Homo sapiens ENSG00000099810   methylthioadenosine phosphorylase
Leishmania braziliensis LbrM.05.0800   methylthioadenosine phosphorylase, putative
Leishmania donovani LdBPK_050830.1   methylthioadenosine phosphorylase, putative
Leishmania infantum LinJ.05.0830   methylthioadenosine phosphorylase, putative
Leishmania major LmjF.05.0830   methylthioadenosine phosphorylase, putative
Leishmania mexicana LmxM.05.0830   methylthioadenosine phosphorylase, putative
Loa Loa (eye worm) LOAG_01831   S-methyl-5'-thioadenosine phosphorylase MTAP
Mus musculus ENSMUSG00000062937   methylthioadenosine phosphorylase
Mycobacterium tuberculosis Rv0535   Probable 5'-methylthioadenosine phosphorylase Pnp (MTA phosphorylase)
Mycobacterium ulcerans MUL_0634   5'-methylthioadenosine phosphorylase
Saccharomyces cerevisiae YLR017W   S-methyl-5-thioadenosine phosphorylase
Schistosoma japonicum Sjp_0213000   ko:K00772 5'-methylthioadenosine phosphorylase [EC2.4.2.28], putative
Schistosoma mansoni Smp_102440   methylthioadenosine phosphorylase
Schistosoma mansoni Smp_028190   methylthioadenosine phosphorylase
Schmidtea mediterranea mk4.006248.03   S-methyl-5'-thioadenosine phosphorylase
Trypanosoma brucei gambiense Tbg972.7.8280   methylthioadenosine phosphorylase, putative
Trypanosoma brucei Tb927.7.7040   methylthioadenosine phosphorylase, putative
Trypanosoma congolense TcIL3000_7_5710   methylthioadenosine phosphorylase, putative
Trypanosoma cruzi TcCLB.506789.280   methylthioadenosine phosphorylase, putative
Trypanosoma cruzi TcCLB.508387.20   methylthioadenosine phosphorylase, putative

Essentiality

Tb927.7.7040 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.7040 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.7040 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.7040 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.7040 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens methylthioadenosine phosphorylase Compounds References
Mus musculus methylthioadenosine phosphorylase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Purine-nucleoside phosphorylase 289 aa 23.2% 276 aa Compounds References
Bos taurus Purine nucleoside phosphorylase 289 aa 24.5% 277 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0968 1 0.5
0.0453 1 0.5
0.1096 0.2887 0.9618
0.1127 0.6098 0.9161
0.0875 0.3279 0.7694
0.0155 1 0.5
0.1134 1 1
0.2141 0.9525 1
0.1063 0.6285 0.952
0.0419 0.2959 0.3801
0.1846 0.3113 1
0.2361 1 1
0.2374 1 1
0.1175 0.2901 1
0.1096 0.2887 0.9618
0.104 0.5673 0.9095
0.1314 0.2921 0.5
0.0236 0.9264 0.5
0.1743 0.3161 1
0.1047 0.6159 0.9082
0.1742 0.4645 1
0.0514 0.3987 1
0.0563 1 1
0.1016 0.3451 1
0.1125 0.2944 1
0.1096 0.2887 0.9618
0.0279 0.4075 1
0.094 0.5465 0.8183
0.0633 1 1
0.0491 1 1
0.1503 0.2939 1
0.0856 0.4793 0.7803
0.0704 0.5123 1
0.1019 0.2541 1
0.032 0.3478 1
0.1071 0.6276 0.9434
0.1893 0.5352 1
0.1303 0.2731 1
0.1016 0.3454 1
0.0172 1 0.5
0.104 0.5673 0.9095
0.0453 1 0.5
0.0172 0.4219 1
0.0172 0.6446 1
0.1057 0.5618 0.9133
0.0852 0.6228 1
0.1067 0.3451 1
0.1009 0.3463 1
0.1019 0.2541 1
0.1567 0.3157 1
0.1071 0.6483 0.913
0.1853 0.3784 1
0.2104 0.9525 1
0.1105 0.6106 0.9158
0.1053 0.7544 0.973
0.2276 1 1
0.1034 0.7267 0.9144
0.12 1 1
0.1055 0.5926 0.9409
0.0856 0.4793 0.7803
0.2495 1 0.5
0.2315 1 1
0.0857 0.4897 0.7662
0.086 0.4774 0.7892
0.0997 0.4378 1
0.1009 0.3474 1
0.1096 0.2887 0.9618
0.094 0.5465 0.8183
0.0172 0.3382 1
0.0172 0.3382 1
0.0563 0.322 0.5155
0.1067 0.3451 1
0.1067 0.7547 0.9735
0.2768 0.6748 1
0.0953 0.5503 0.8181
0.0867 0.4793 0.7911
0.1466 0.2774 1
0.1019 0.2541 1
0.1096 0.2887 0.9618
0.0172 0.6914 1
0.0172 0.3382 1
0.1446 0.4525 1
0.0886 1 1
0.1019 0.2541 1
0.1107 0.6497 0.9747
0.2768 0.6748 1
0.1019 0.2541 1
0.1067 0.3451 1
0.1042 0.617 0.9099
0.1839 0.9252 1
0.1138 0.2634 0.9528
0.104 0.5673 0.9095
0.104 0.4628 0.9108
0.1027 0.3847 1
0.0862 0.3704 0.7643
0.2104 0.9525 1
0.1357 0.2878 1
0.1976 0.3088 1
0.0633 1 1
0.098 0.3983 1
0.0172 1 1
0.096 0.3473 1
0.1097 0.576 0.942
0.0559 0.3201 0.5055
0.286 0.5763 0.5
0.1567 0.3157 1
0.1099 0.6107 0.9176
0.0331 0.9348 0.5
0.1019 0.2541 1
0.0172 1 0.5
0.0588 0.7663 1
0.1385 0.461 0.5
0.0561 0.6404 1
0.0561 0.4018 0.5181
0.0857 0.4887 0.7699
0.1067 0.7546 0.9472
0.0997 0.4378 1
0.1044 0.7368 0.9501
0.0282 0.2742 1
0.1147 0.5194 1
0.0419 0.2544 1
0.1567 0.3157 1
0.1215 1 0.5
0.0172 0.6446 1
0.123 0.3766 0.5
0.2481 1 0.5
0.1019 0.2541 1
0.0204 1 0.5
0.1503 0.2939 1
0.1832 0.3167 1
0.0603 0.3249 1
0.2296 1 1
0.0172 1 1
0.2641 0.2892 1
0.1016 0.3451 1
0.1044 0.7368 0.9501
0.0852 0.6228 1
0.1003 0.9578 0.9537
0.1009 0.3474 1
0.0347 0.4262 1
0.0422 0.3056 0.6052
0.0564 0.4012 0.5182
0.1099 0.6196 0.9288
0.1567 0.3157 1
0.0712 0.4912 1
0.0852 0.4897 0.7681
0.1073 0.748 0.9567
0.1009 0.3463 1
0.0172 0.4062 1
0.2623 0.8455 1
0.2129 0.8656 0.5
0.042 1 1
0.1872 0.5427 1
0.0624 0.293 1
0.0564 0.4028 0.5229
0.148 0.2726 1
0.0859 0.3435 0.8037
0.1019 0.2541 1
0.0872 0.6346 1
0.105 0.747 0.9582
0.1122 0.611 0.9179
0.1995 0.3349 1
0.1023 0.7477 0.9574
0.1047 0.7444 0.96
0.1071 0.5536 0.9172
0.1096 0.2887 0.9618
0.104 0.3554 1
0.2233 1 1
0.1919 1 1
0.0563 0.3205 0.4902
0.0886 1 1
0.094 0.5465 0.8183
0.2641 0.2892 1
0.086 0.6292 1
0.2623 0.8455 1
0.1005 0.44 1
0.1067 0.3451 1
0.0849 0.4784 1
0.1046 0.7384 0.9522
0.1078 0.6091 1
0.1118 0.6534 0.9722
0.1105 0.6182 0.927
0.0311 1 0.5
0.1096 0.2887 0.9618
0.0997 0.4378 1
0.0701 0.5154 0.652
0.1138 0.2634 0.9528
0.0852 0.4893 0.768
0.0347 0.4262 1
0.1012 0.7536 1
0.1044 0.7368 0.9501
0.1016 0.3451 1
0.1231 0.3323 1
0.1019 0.2541 1
0.1314 0.2921 0.5
0.0848 0.4805 0.7783
0.0858 0.4796 0.7912
0.1722 0.3472 1
0.1096 0.2887 0.9618
0.1028 0.7561 1
0.0947 0.2916 0.9903
0.186 0.5739 1
0.1067 0.3451 1
0.1265 0.7235 1
0.0633 1 1
0.213 1 0.5
0.1097 0.6269 0.9419
0.0282 0.2742 1
0.1816 0.3041 1
0.0473 0.8483 1
0.0172 0.3495 1
0.111 0.6438 0.9625
0.0947 0.5385 0.7968
0.1105 0.6106 0.9158
0.1771 0.3379 1
0.0997 0.4378 1
0.096 0.3473 1
0.2141 0.9525 1
0.0284 1 1
0.0563 0.322 0.5155
0.104 0.5673 0.9095
0.2768 0.6748 1
0.2609 1 1
0.0172 0.3382 1
0.0172 1 0.5
0.1078 0.6067 0.951
0.0855 0.4775 0.7892
0.0964 0.5333 1
0.2315 1 1
0.0172 1 0.5
0.1019 0.2541 1
0.0236 0.9264 0.5
0.0977 0.5421 0.8134
0.0169 0.5134 0.5
0.1919 1 0.5
0.05 0.3744 1
0.201861 1 0.5
0.2301 1 1
0.0868 0.6397 1
0.2323 0.6798 1
0.1074 0.6361 0.954
0.1056 0.7454 0.9526
0.0795 0.4563 1
0.0997 0.4378 1
0.1092 0.6267 0.9008
0.0236 0.9264 0.5
0.1042 0.6259 0.9412
0.0172 0.6914 1
0.0862 0.3704 0.7643
0.1034 0.3331 0.9105
0.1063 0.7781 0.9924
0.0416 0.4071 1
0.173 0.3172 1
0.1456 0.4663 0.5
0.096 0.3473 1
0.1019 0.2541 1
0.1016 0.3454 1
0.1034 0.7563 1
0.0845 1 1
0.104 0.3554 1
0.2641 0.2892 1
0.2208 0.2856 1
0.1137 0.445 0.5
0.1048 0.7559 1
0.1985 1 1
0.0172 0.2848 0.5
0.0867 0.482 0.7823
0.0422 0.2566 0.811
0.1313 0.2751 1
0.0423 0.3018 0.8109
0.0172 1 0.5
0.0997 0.4378 1
0.234 1 1
0.0964 0.5333 1
0.0858 0.6112 1
0.0876 0.478 0.7787
0.0172 1 0.5
0.0865 0.4749 0.7889
0.1766 0.3219 1
0.1211 1 1

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei brucei ( 1 )

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.7.7040 (Trypanosoma brucei), methylthioadenosine phosphorylase, putative
Title for this comment
Comment